產(chǎn)品英文名稱：LONZA 12-719Q DMEM:F12 1:1Mixture with HEPES,L-Gln,Basal medium

產(chǎn)品中文俗稱：DMEM:F12 1:1Mixture with HEPES,L-Gln,基礎(chǔ)培養(yǎng)基

12-719Q DMEM:F12 1Mixture with HEPES,L-Gln,基礎(chǔ)培養(yǎng)基描述：

DMEM:F12 combines the richness of F12 with the higher component concetrations of DMEM. This medium is well-suited for clonal density cultures. 
All BioWhittaker™ Cell Culture Media products are manufactured in accordance with cGMP regulations. The formulations used for standard liquid media products are those recommended by the Tissue Culture Association.

絕大多數(shù)培養(yǎng)基是建立在平衡鹽溶液(BSS)基礎(chǔ)上，添加了氨基酸、維生素和其它與血清中濃度相似的營(yíng)養(yǎng)物質(zhì)。最廣泛應(yīng)用的培養(yǎng)基是Eearle`s MEM 的混合物，其中含有13種必須氨基酸、8種維生素。而Ham`s F12 也包括非必須氨基酸，維生素的范圍亦很廣，另外常規(guī)含有無(wú)機(jī)鹽和代謝添加劑（例如核苷酸）。MEM/F12 這兩種培養(yǎng)基各取1/2，形成神經(jīng)生物學(xué)最通用的培養(yǎng)基。Dulbecco`s改良培養(yǎng)基——DMEM，現(xiàn)應(yīng)用于快速生長(zhǎng)的細(xì)胞，同MEM含有相同的營(yíng)養(yǎng)成分，但濃度高出2~4倍。選擇某種培養(yǎng)基，應(yīng)仔細(xì)了解成分表，應(yīng)知道大多數(shù)情形下培養(yǎng)基都有不足。例如，有些培養(yǎng)基在氨基酸中包括有谷氨酸，而這種培養(yǎng)基雖廣泛用于神經(jīng)生物學(xué)領(lǐng)域，但它對(duì)某些對(duì)谷氨酸敏感的可能有細(xì)胞外毒性損傷的神經(jīng)元而言，則并非最佳選擇，特別是如果神經(jīng)元生長(zhǎng)在缺乏膠質(zhì)的環(huán)境中時(shí)。F12中含有硫酸亞鐵，據(jù)報(bào)道也有神經(jīng)毒效應(yīng)。

12-719Q DMEM:F12 1Mixture with HEPES,L-Gln,Product use：

DMEM combined with Hams F12 has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

LONZA DMEM:F12 1:1Mixture with HEPES,L-Gln,Basal medium 系列產(chǎn)品介紹：

LONZA  12-719F  DMEM:F12 1:1Mixture with HEPES, L-Gln基礎(chǔ)培養(yǎng)基    500mL/瓶  
LONZA 12-719Q DMEM:F12 1:1Mixture with HEPES, L-Gln基礎(chǔ)培養(yǎng)基    1000mL/瓶

Lonza DMEM:F12 培養(yǎng)基相關(guān)產(chǎn)品介紹：

 DMEM培養(yǎng)基：高糖更適合高密度懸浮細(xì)胞培養(yǎng)，低糖適于依賴性貼壁細(xì)胞培養(yǎng)。

12-604F  DMEM 4.5 g/L Glucose w/ L-Gln   500 ml

12-604Q  DMEM 4.5 g/L Glucose w/ L-Gln     1L

12-741F  DMEM 4.5 g/L Glucose w/ L-Gln,w/o Pyr  500 ml

15-604D  DMEM 4.5 g/L Glucose Powder, w/ L-Gln   10L

12-614F  DMEM 4.5 g/L Glucose w/o L-Gln    500 ml

12-614Q  DMEM 4.5 g/L Glucose w/o L-Gln     1 L

12-914F  DMEM 4.5 g/L Glucose w/o L-Gln HybridomaTest 500ml

12-917F  DMEM 4.5 g/L Glucose w/o L-Gln,Phenol Red    500 ml

12-709F  DMEM 4.5 g/L Glucose w/o L-Gln, w/HEPES      500 ml

12-733F  DMEM 4.5 g/L Glucose w/o L-Gln or Pyr        500ml

12-733Q  DMEM 4.5 g/L Glucose w/o L-Gln or Pyr       1 L

12-707F  DMEM 1.0 g/L Glucose w/o L-Gln    500ml

12-708F  DMEM 1.0 g/L Glucose w/o L-Gln,w/HEPES    500 ml

08-332B   DMEM 4.5 g/L Glucose w/o L-Gln   10 L bag

08-332F   DMEM 4.5 g/L Glucose w/o L-Gln   20 L bag

15-604F   DMEM 4.5 g/L Glucose,Powder, w/ L-Gln      50L

15-614D   DMEM 4.5 g/L Glucose,Powder wo/L-Gln       10L

DMEM:F12培養(yǎng)基：適于克隆密度的培養(yǎng)

12-719F   DMEM:F12 1:1Mixture with HEPES, L-Gln   500 ml

12-719Q   DMEM:F12 1:1Mixture with HEPES, L-Gln    1 L

04-687Q   DMEM:F12 1:1 wo HEPES      1 L