英文名稱(chēng)：Recombinant Mouse Fibroblast Growth Factor-basic
中文簡(jiǎn)稱(chēng)：【mFGF 2】重組小鼠堿性成纖維細(xì)胞生長(zhǎng)因子
Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重組小鼠堿性成纖維細(xì)胞生長(zhǎng)因子產(chǎn)品描述：
堿性成纖維細(xì)胞生長(zhǎng)因子（basic fibroblast growth factor，bFGF）是成纖維細(xì)胞生長(zhǎng)因子蛋白（fibroblast growth factors，F(xiàn)GFs）家族中的一員。目前已發(fā)現(xiàn)至少22種FGF，其中N3F-1（aFGF）、FGF-2（bFGF）及FGF-7的研究較為深入。bFGF是哺乳動(dòng)物和人體中一種非常微量的活性物質(zhì)，具有廣泛的生理功能和重要的臨床應(yīng)用價(jià)值。bFGF不僅能刺激新生血管形成，參與創(chuàng)傷愈合和組織再生，促進(jìn)胚胎組織發(fā)育和分化等，此外還與腫瘤的發(fā)生發(fā)展密切相關(guān)。近年來(lái)，重組bFGF已被應(yīng)用于臨床治療創(chuàng)傷、潰瘍及神經(jīng)系統(tǒng)等疾病。
小鼠堿性成纖維細(xì)胞生長(zhǎng)因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有145個(gè)氨基酸，分子量為16320Da。該小鼠堿性成纖維細(xì)胞生長(zhǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重組小鼠堿性成纖維細(xì)胞生長(zhǎng)因子產(chǎn)品使用須知：
溶解：提供的小鼠堿性成纖維細(xì)胞生長(zhǎng)因子 (mFGF 2)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含小鼠堿性成纖維細(xì)胞生長(zhǎng)因子 (mFGF 2)的5mM NaP（pH 7.5）和50mM NaCl溶液中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：小鼠堿性成纖維細(xì)胞生長(zhǎng)因子 (mFGF 2)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重組小鼠堿性成纖維細(xì)胞生長(zhǎng)因子產(chǎn)品英文描述：
Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.
Introduction
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
Description
Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.3kDa. The FGF-2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance （物理特征）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
FGF-b was lyophilized from 5mM Na2PO4, pH7.5 and 50mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor b in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS.
Biological Activity （生物活性）
The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be 0.1-0.16ng/ml.
Protein content （蛋白質(zhì)含量）
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard.
